01 January 2015 by Michael Yeager, Kurt R Stenmark

Hypoxia promotes the emergence of cells co-expressing leukocytic (CD11b) and the myofi broblast marker -SM-actin

AI00129.jpg
Yeager ME,Frid MG,Stenmark KR.Progenitor cells in pulmonary vascular remodeling. [Internet]. 2011 ;1:3. Available from:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198626/

Hypoxia promotes the emergence of cells co-expressing leukocytic (CD11b) and the myofi broblast marker -SM-actin. (a) A robust infl ux of CD11b+ cells (red) in the PA adventitia of chronically hypoxic rats correlates with a signifi cant number of -SMA+ myofi broblasts in that region. A Adventitia; M Media. (b) Double-label immunofl uorescent staining for CD11b (red) and -SMA (green), followed by deconvolution confocal microscopy analysis of the 'boxed' area in A, demonstrate cells that co-express both markers (arrowheads), or express only one of the markers (-SMA, single arrow or CD11b, triple arrows). (c) Signifi cant numbers of -SMA+ myofi broblasts (brown staining in the adventitia (labeled ('A')) are present in distal PAs of the chronically hypoxic calves. (d) Double-label immunofl uorescent staining, followed by deconvolution confocal microscopy, demonstrate that some of the -SMA+ myofi broblasts (green) co-express a macrophage marker CD68 (red) (arrowheads). DAPI (blue) labels cell nuclei. M, media; A, adventitia. Scale bars: 50 m (a, c); 5 m (b, d). (Adapted from: Frid et al.)

 

Additional keywords: fibroblast,leukocytes,

Science that starts with a hello.

Through global collaboration, we make today's work, tomorrow's possibility.

Join the PVRI
standard-example-image.jpg