Inhibition of mutant PDGFR-b (D849N) phosphorylation by imatinib. Wild type and mutant (D849N) murine embryonic fibroblasts (MEFs) were pre-incubated with or without imatinib (3M) for 3 hours and stimulated with PDGF-BB (20ng/ml, 10 min.). Immunoprecipitation (IP)/ Western blot was performed to obtain relative amounts of PDGFR-b (lower panel) followed by membrane stripping and reblotting for phosphorylated PDGFR-b (upper bands). The phosphorylated- and total PDGFR-b are shown. WT-wild type; mut- mutant; pPDGFR-b- pan-phosphorylated PDGFR-b.
Additional keywords: imatinib,mouse,PDGF,fibroblast,D849N