01 January 2015 by Bhola Kumar Dahal, Norbert Weissmann, Werner Seeger, Ralph T. Schermuly

Inhibition of mutant PDGFR-b (D849N) phosphorylation by imatinib

Schermuly {RT},Dahal {BK},Pullamsetti {SS},Ghofrani {HA},Seeger W,Heuchel R,Wilhelm J,Weissmann N,Grimminger F.Hypoxic pulmonary hypertension in mice with constitutively active platelet-derived growth factor receptor-. [Internet]. 2011 ;1:259. Available from:http://www.pulmonarycirculation.org/text.asp?2011/1/2/259/83448

Inhibition of mutant PDGFR-b (D849N) phosphorylation by imatinib. Wild type and mutant (D849N) murine embryonic fibroblasts (MEFs) were pre-incubated with or without imatinib (3M) for 3 hours and stimulated with PDGF-BB (20ng/ml, 10 min.). Immunoprecipitation (IP)/ Western blot was performed to obtain relative amounts of PDGFR-b (lower panel) followed by membrane stripping and reblotting for phosphorylated PDGFR-b (upper bands). The phosphorylated- and total PDGFR-b are shown. WT-wild type; mut- mutant; pPDGFR-b- pan-phosphorylated PDGFR-b.


Additional keywords: imatinib,mouse,PDGF,fibroblast,D849N

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