Muscularization of pulmonary vessels in hypoxic mutant (D849N) mice receiving imatinib. Wild type and D849N mice were exposed to hypoxia for 35 days or remained in normoxia throughout (normoxic control). Hypoxic mice (n=10) received imatinib orally by gavage from day 21 to day 35 at a dose of 100 mg kg 1 BW. Hypoxic control animals received placebo (n=10). Lung sections were immunostained for a-SMC actin (arrow) and vWF (arrow head) followed by pulmonary vascular morphometry. A total of 80 to 100 intra-acinar vessels were analyzed in each lung. (a) Representative photomicrographs are shown. (b) Proportions of non-muscularized (N), partially muscularized (P), or fully muscularized (M) pulmonary vessels, as percentage is given. Bar represents meanSEM. #1.6 times higher than the fully muscularized vessels in hypoxic WT. *P<0.05 vs corresponding normoxic control; P<0.05 vs corresponding hypoxic control. Scale bar=20 mm.
Additional keywords: imatinib,mouse,PDGF,pulmonary vascular remodeling