04 February 2019 by Jun Yang

The inflammatory factors involved in the pathogenesis of pulmonary arterial hypertension in systemic lupus erythematosus

Introduction

Systemic lupus erythematosus (SLE) is the most common connective tissue diseases associated with pulmonary arterial hypertension (PAH) in China. 4% of Lupus patients suffer from PAH which is one of the leading causes of death for SLE. The pathologic changes and underline mechanism lead to the pulmonary vascular remodelling in SLE patients remains to be identified.

Method

SNPs selected from TGF-β pathway genes (BMPR2, ALK1, Endoglin and SMAD9) were detected in SLE-PAH patients from Peking Union Medical College Hospital registry to explore genetic susceptibility for PAH in SLE. The level of autoantibodies of BMP receptors in serum from patients with SLE-PAH and controls (SLE patients without PAH and healthy volunteers) were detected via enzyme linked immunosorbent assay assays. The inflammatory factors were examined by immunoblotting in pulmonary arterial endothelial cells (PAECs) stimulated with bacteria toxin. Finally, we analysed the specific immune cell(s) involved in SLE-PAH by a comparation of cellular components in peripheral blood collected from patients prior to the first administration and after treatment of glucocorticoids and vasodilators.

Results

We identified that the autoantibodies of BMP receptors were higher in the serum of SLE-PAH patients compared with controls. Furthermore, the expression level of certain inflammatory factors in PAECs was affected by bacteria toxin. The number of granulocytes was elevated in the SLE-PAH patients after treatment, which indicated that granulocytes may participate in the develop of PAH in SLE patients.

Conclusion

Our results revealed mechanism of PAH onset in SLE, implied certain pro-inflammatory factors were involved in the pathogenesis of SLE-PAH.

 

About the author


profile picture of Jun Yang

Jun Yang

Professor

Chinese Academy of Medical Sciences

China

Key Contributors

Yanjiang Xing 1 , Meijun Zhou 1,Junyan Qian 2, Xin Zhao 1, Hongxian Liu 1, Mengtao Li 2, Xiaofeng Zeng 2, Jun Yang 1 : 1 State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China. 2 Department of Rheumatology, Peking Union Medical College Hospital, Beijing, China.


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