The temporospatial development of the pulmonary vasculature is poorly understood. A better understanding of the cellular interactions that specify normal development of the pulmonary vasculature could lead to new therapies for conditions such as pulmonary hypertension. We aim to characterize vascular cells in murine embryonic lungs as they progress through developmental stages.
Cdh5-CreER ROSA-TdTm mice were injected with tamoxifen at E8.5, to mark the progeny of early vascular progenitor cells by genetic lineage tracing. Lungs were harvested at E14.5 and analyzed by immunofluorescence (IF) staining, FACS analysis and single cell RNA sequencing.
We detected TdTm+ cells with morphologies and distributions consistent with venous, arterial and capillary endothelial cells. This suggests that there is a multipotent vascular precursor population as early as E8.5. Tm4sf1 was identified as a potential marker of multipotent vascular precursors in single cell transcriptomic data.
Our objective is to further characterize the expression of Tm4sf1 in these progenitor cells and its role in the development of mature pulmonary vasculature.